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gapdh fitzerald industries international  (Bethyl)


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    Bethyl gapdh fitzerald industries international
    Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
    Gapdh Fitzerald Industries International, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 276 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gapdh fitzerald industries international/product/Bethyl
    Average 96 stars, based on 276 article reviews
    gapdh fitzerald industries international - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "Repair of protein-linked DNA double strand breaks: Using the adenovirus genome as a model substrate in cell-based assays"

    Article Title: Repair of protein-linked DNA double strand breaks: Using the adenovirus genome as a model substrate in cell-based assays

    Journal: DNA repair

    doi: 10.1016/j.dnarep.2018.12.001

    Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; RPA32, the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
    Figure Legend Snippet: Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; RPA32, the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).

    Techniques Used: Infection, Labeling, Immunoprecipitation, Western Blot, Binding Assay, Virus, Phospho-proteomics, Two-Dimensional Gel Electrophoresis, SDS Page, Incubation, DNA Sequencing, Derivative Assay

    Variation in TP-oligo size during dl1004 vs. WT Ad5 infection. HeLa cells were infected with dl1004 (A) or WT (B), harvested at the indicated time post infection, and TP was immunoprecipitated with the indicated antibody before being labeled with [α-32P]-cordycepin triphosphate by TdT. Nbs1, Nijmegen breakage syndrome protein 1 (Nibrin); DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; h.p.i., hours post infection.
    Figure Legend Snippet: Variation in TP-oligo size during dl1004 vs. WT Ad5 infection. HeLa cells were infected with dl1004 (A) or WT (B), harvested at the indicated time post infection, and TP was immunoprecipitated with the indicated antibody before being labeled with [α-32P]-cordycepin triphosphate by TdT. Nbs1, Nijmegen breakage syndrome protein 1 (Nibrin); DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; h.p.i., hours post infection.

    Techniques Used: Infection, Immunoprecipitation, Labeling, Binding Assay

    2.1. Antibodies
    Figure Legend Snippet: 2.1. Antibodies

    Techniques Used:



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    gapdh fitzerald industries international  (Bethyl)
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    Bethyl gapdh fitzerald industries international
    Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
    Gapdh Fitzerald Industries International, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gapdh fitzerald industries international/product/Bethyl
    Average 96 stars, based on 1 article reviews
    gapdh fitzerald industries international - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier

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    Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; RPA32, the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).

    Journal: DNA repair

    Article Title: Repair of protein-linked DNA double strand breaks: Using the adenovirus genome as a model substrate in cell-based assays

    doi: 10.1016/j.dnarep.2018.12.001

    Figure Lengend Snippet: Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; RPA32, the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).

    Article Snippet: Additional antibodies used in this study: Protein Target Antibody Source Catalog # Adenovirus Gift from Arnold Levine, Institute for Advanced Study Monoclonal DBP #B6 GAPDH Fitzerald Industries International (RDI Division) RDI-TRK5G4- 6C5 RPA32 Gift from Thomas Melendy, State University of New York at Buffalo _____ RPA32 (S4/S8) Bethyl A300-245 A Nbs1 Novus Biologicals NB 100-143 Mre11 GeneTex GTX70212 CtIP Santa Cruz Biotechnologies sc-5970 BRCA1 Calbiochem OP92 HA-tag Santa Cruz Biotechnologies sc-7392 Open in a separate window 2.1.

    Techniques: Infection, Labeling, Immunoprecipitation, Western Blot, Binding Assay, Virus, Phospho-proteomics, Two-Dimensional Gel Electrophoresis, SDS Page, Incubation, DNA Sequencing, Derivative Assay

    Variation in TP-oligo size during dl1004 vs. WT Ad5 infection. HeLa cells were infected with dl1004 (A) or WT (B), harvested at the indicated time post infection, and TP was immunoprecipitated with the indicated antibody before being labeled with [α-32P]-cordycepin triphosphate by TdT. Nbs1, Nijmegen breakage syndrome protein 1 (Nibrin); DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; h.p.i., hours post infection.

    Journal: DNA repair

    Article Title: Repair of protein-linked DNA double strand breaks: Using the adenovirus genome as a model substrate in cell-based assays

    doi: 10.1016/j.dnarep.2018.12.001

    Figure Lengend Snippet: Variation in TP-oligo size during dl1004 vs. WT Ad5 infection. HeLa cells were infected with dl1004 (A) or WT (B), harvested at the indicated time post infection, and TP was immunoprecipitated with the indicated antibody before being labeled with [α-32P]-cordycepin triphosphate by TdT. Nbs1, Nijmegen breakage syndrome protein 1 (Nibrin); DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; h.p.i., hours post infection.

    Article Snippet: Additional antibodies used in this study: Protein Target Antibody Source Catalog # Adenovirus Gift from Arnold Levine, Institute for Advanced Study Monoclonal DBP #B6 GAPDH Fitzerald Industries International (RDI Division) RDI-TRK5G4- 6C5 RPA32 Gift from Thomas Melendy, State University of New York at Buffalo _____ RPA32 (S4/S8) Bethyl A300-245 A Nbs1 Novus Biologicals NB 100-143 Mre11 GeneTex GTX70212 CtIP Santa Cruz Biotechnologies sc-5970 BRCA1 Calbiochem OP92 HA-tag Santa Cruz Biotechnologies sc-7392 Open in a separate window 2.1.

    Techniques: Infection, Immunoprecipitation, Labeling, Binding Assay

    2.1. Antibodies

    Journal: DNA repair

    Article Title: Repair of protein-linked DNA double strand breaks: Using the adenovirus genome as a model substrate in cell-based assays

    doi: 10.1016/j.dnarep.2018.12.001

    Figure Lengend Snippet: 2.1. Antibodies

    Article Snippet: Additional antibodies used in this study: Protein Target Antibody Source Catalog # Adenovirus Gift from Arnold Levine, Institute for Advanced Study Monoclonal DBP #B6 GAPDH Fitzerald Industries International (RDI Division) RDI-TRK5G4- 6C5 RPA32 Gift from Thomas Melendy, State University of New York at Buffalo _____ RPA32 (S4/S8) Bethyl A300-245 A Nbs1 Novus Biologicals NB 100-143 Mre11 GeneTex GTX70212 CtIP Santa Cruz Biotechnologies sc-5970 BRCA1 Calbiochem OP92 HA-tag Santa Cruz Biotechnologies sc-7392 Open in a separate window 2.1.

    Techniques: